Periodic Acid 0.5 %
In PAS staining, the periodic acid solution oxidizes the 1,2-glycol groups in the polysaccharides and glycoproteins to aldehyde groups. The tissue is then treated with Schiff's reagent, which binds to the newly formed aldehyde groups to produce an intense purple or magenta stain. This staining allows the identification of structures such as basement membranes, mucus-producing glands, glycogen in liver and muscle tissue, and other carbohydrate-containing structures under the microscope.
Article no.: 11167
Oxidation of tissue samples
product information
Relevant Incredients:
• Periodic Acid
Instructions / Protocol / Recommendations
Verwendung:
The 0.5% periodic acid solution is used in in vitro diagnostics for the oxidation of tissue samples, especially in the periodic acid Schiff reaction (PAS) in histology. Treatment with periodic acid solution forms aldehyde groups in polysaccharides and glycoproteins, which subsequently produce intense staining with Schiff's reagent. This allows microscopic identification of carbohydrate-containing structures such as basement membranes, mucus-producing glands, glycogen in liver and muscle tissue.
Prinzip:
Periodic acid (0.5 %) is an oxidative solution used in in vitro diagnostics to visualize tissue or cell structures. The functional principle is based on the selective oxidation of carbohydrate structures such as polysaccharides or glycoproteins in tissue or cell samples. This oxidation results in the formation of aldehyde groups, which subsequently react with specific dyes or antibodies to visualize the desired structures. This method allows high sensitivity and specificity in the identification of biological structures and facilitates the analysis of tissue or cell samples.
Verfahren:
Periodic acid (0.5 %) is an oxidative solution used in in vitro diagnostics to visualize tissue or cell structures. The functional principle is based on the selective oxidation of carbohydrate structures such as polysaccharides or glycoproteins in tissue or cell samples. This oxidation results in the formation of aldehyde groups, which subsequently react with specific dyes or antibodies to visualize the desired structures. This method allows high sensitivity and specificity in the identification of biological structures and facilitates the analysis of tissue or cell samples.
Hazard and safety instructions
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