Paraformaldehyde (PFA) 4 %, in PBS pH 11.0
Paraformaldehyde (PFA) 4 % in PBS pH 11.0 is a specially formulated fixative solution that excels in its applications in histology, medical diagnostics and life sciences. It is particularly suitable for immunohistochemistry where improved antibody penetration is required, especially for densely cross-linked or thick tissue sections.
The fixing solution contains 4% paraformaldehyde in a phosphate-buffered saline (PBS) solution with a pH of 11.0. The alkaline environment reduces cross-linking and fixation of tissue, allowing better antibody accessibility to intracellular structures and epitopes. This may help improve the sensitivity of antibody binding and the quality of the results obtained.
Chemically, paraformaldehyde is a polymer of formaldehyde that depolymerizes to formaldehyde in aqueous solution. Combined with the buffering effect of PBS, this fixing solution helps to keep the pH stable during fixation.
It is important to carefully consider and optimize the use of PFA 4% in PBS pH 11.0 to avoid potential adverse effects on tissue morphology and antibody binding sensitivity. Therefore, this fixative solution should only be used after appropriate validation and adaptation to the specific experiment or question.
The fixing solution contains 4% paraformaldehyde in a phosphate-buffered saline (PBS) solution with a pH of 11.0. The alkaline environment reduces cross-linking and fixation of tissue, allowing better antibody accessibility to intracellular structures and epitopes. This may help improve the sensitivity of antibody binding and the quality of the results obtained.
Chemically, paraformaldehyde is a polymer of formaldehyde that depolymerizes to formaldehyde in aqueous solution. Combined with the buffering effect of PBS, this fixing solution helps to keep the pH stable during fixation.
It is important to carefully consider and optimize the use of PFA 4% in PBS pH 11.0 to avoid potential adverse effects on tissue morphology and antibody binding sensitivity. Therefore, this fixative solution should only be used after appropriate validation and adaptation to the specific experiment or question.
Article no.: 13562
Fixation of tissue samples
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UN-Nummer: 0000
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Lagerung: 4 … 8 °C
Haltbarkeit: 10 Tage
product information
Relevant Incredients:
• Di-sodium hydrogen phosphate dihydrate
• Sodium chloride
• Paraformaldehyde
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