PBS Buffer - Concentrate
This PBS buffer concentrate is used in numerous applications in cell culture, molecular biology, immunohistochemistry and protein extraction. It provides a stable environment for biological samples by maintaining physiological pH and osmotic pressure.
The product finds particular suitability in the preparation of cell washes and cell suspensions. The phosphate and salt components act synergistically to stabilize cell membranes and protect protein conformations.
The chemical properties of PBS buffer concentrate are based on the mixture of di-sodium hydrogen phosphate, potassium di-hydrogen phosphate and sodium chloride. The concentrate has a pH of 5.9 to 6.1, which can be adjusted to a physiological pH of 7.0 to 7.4 by diluting in various ratios.
The operation of the product relies on the buffering capacity of the phosphate groups. They compensate for fluctuations in pH by releasing or absorbing protons. The ionic strength, mainly provided by sodium chloride, regulates the osmotic pressure and contributes to the preservation of cell morphology.
The product finds particular suitability in the preparation of cell washes and cell suspensions. The phosphate and salt components act synergistically to stabilize cell membranes and protect protein conformations.
The chemical properties of PBS buffer concentrate are based on the mixture of di-sodium hydrogen phosphate, potassium di-hydrogen phosphate and sodium chloride. The concentrate has a pH of 5.9 to 6.1, which can be adjusted to a physiological pH of 7.0 to 7.4 by diluting in various ratios.
The operation of the product relies on the buffering capacity of the phosphate groups. They compensate for fluctuations in pH by releasing or absorbing protons. The ionic strength, mainly provided by sodium chloride, regulates the osmotic pressure and contributes to the preservation of cell morphology.
Article no.: 11170
Preparation of buffer solutions
product information
Relevant Incredients:
• Di-sodium hydrogen phosphate dihydrate
• Potassium dihydrogen phosphate
• Sodium chloride