Nigrosin 10 %, aqueous
The 10% nigrosine solution is used extensively in bioscientific and diagnostic procedures, especially as a negative staining agent in microscopy. It is particularly effective in studies where the highlighting of cell boundaries and the differentiation of living and dead cells is required.
The solution has the advantage of not penetrating living cells, making it an ideal tool for cell viability determination. Likewise, its strong contrasting properties make it useful in histology to visualize structures more clearly.
Nigrosine, an acidic azo dye, has the chemical property of binding to and labeling the cell membrane without penetrating the cell. This allows clear delineation of cell contours when viewed under a microscope.
In practice, nigrosine is applied to a cell sample. Since the dye does not penetrate living cells, they remain bright, while dead cells and background structures appear dark. This allows easy differentiation between living and dead cells and improves the visibility of cell boundaries.
The solution has the advantage of not penetrating living cells, making it an ideal tool for cell viability determination. Likewise, its strong contrasting properties make it useful in histology to visualize structures more clearly.
Nigrosine, an acidic azo dye, has the chemical property of binding to and labeling the cell membrane without penetrating the cell. This allows clear delineation of cell contours when viewed under a microscope.
In practice, nigrosine is applied to a cell sample. Since the dye does not penetrate living cells, they remain bright, while dead cells and background structures appear dark. This allows easy differentiation between living and dead cells and improves the visibility of cell boundaries.
Article no.: 14233
Use as laboratory reagent
product information
Relevant Incredients:
• Nigrosine (C.I.: 50420)