The Michaelis buffer with a pH value of 8.6 is used in various scientific and medical fields. One main application is electrophoresis, a process that separates biomolecules according to their electrical charge. With its stable alkaline environment, the buffer promotes the separation efficiency and reproducibility of electrophoresis.

In medical diagnostics and life sciences, the buffer shows particular suitability for serum protein electrophoresis and immunoelectrophoresis. Its pH stability ensures optimal conditions for these special procedures.

Chemically, Michaelis buffer is a mixture of sodium 5,5-diethyl barbiturate, barbital, sodium benzoic acid methyl ester and sodium azide dissolved in distilled water. These components interact with each other to create a stable pH environment and make the buffer resistant to changes caused by the addition of acids or bases.

The operation of this buffer is based on its ability to stabilize the pH of a solution. This property is critical for many laboratory applications where a constant pH environment is important, making the Michaelis buffer an indispensable tool in life science research.