Crystal violet 4%, alcoholic is a solution containing crystal violet in denatured ethanol as a solvent. The product is primarily used in microbiology and histology.

Main applications include Gram staining of bacteria, where crystal violet is used to stain Gram-positive bacteria. The staining is based on the binding of crystal violet to peptidoglycan in the cell walls of bacteria. In histology, crystal violet is used to stain cell nuclei, allowing them to be visualized in microscopic examinations.

The chemical functioning of the solution is based on the interaction between crystal violet and the structures to be stained. Crystal violet, a basic dye, binds to acidic components of cells, such as nucleic acids or peptidoglycan. The presence of denatured ethanol promotes the solubility of the dye and contributes to the intensity and stability of the staining.

Crystal Violet 4 %, alcoholic is particularly suitable for the examination of bacteria by Gram staining and for the visualization of cell nuclei in histological preparations. The solution enables high-contrast staining and provides reliable results in the identification and differentiation of cell structures and bacteria.