Rhinehart & Abu'l Haj colloidal iron solution is a proven tool for the identification and differentiation of acidic mucopolysaccharides in histological specimens. It is widely used in medical diagnostics and life sciences, especially in histology.

The stock solution consists of iron(III) chloride dissolved in a combination of distilled water and glycerol. Ammonia plays a crucial role in the preparation of the solution by allowing the formation of a colloidal suspension of iron hydroxide. Acetic acid, supplied separately, serves as an acidic buffer and contributes to optimal staining.

The chemical reaction between the colloidal iron in the stock solution and the acidic mucopolysaccharides in the tissue samples produces a specific staining that is optimized by the addition of acetic acid. The results of this staining are particularly useful in ultrastructural research to visualize specific cell structures.

By combining colloidal iron and acetic acid, this kit provides reliable and efficient staining that allows clear visualization of acidic mucopolysaccharides in histological samples. These properties make the kit a valuable tool in histological research and diagnostics.