Berlinerblau staining solution 2.5% is a higher concentration version of the traditional solution and is used in histology, medical diagnostics and life sciences. It enables the identification of iron deposits in biological samples, with the increased concentration providing greater sensitivity in the detection of iron.

The main advantage of this particular solution is its increased sensitivity in the detection of iron deposits in tissue samples. This can be particularly important in the investigation of diseases in which small amounts of iron are involved.

Chemically, Berlinerblau Staining Solution 2.5% consists of a 5% potassium hexacyanoferrate(II) solution and a 5% hydrochloric acid solution specifically optimized for iron detection reactions. The increased concentration of potassium hexacyanoferrate(II) and hydrochloric acid allows for better reaction speed and sensitivity in the formation of Berlin blue, the ferric hexacyanoferrate(II) complex.

The operation of Berlin blue staining solution 2.5% is based on the formation of the Berlin blue complex, which is composed of ferric ions and hexacyanoferrate(II) ions. The increased concentration of this solution enables more efficient and sensitive detection of iron deposits in tissue samples, which is relevant for research and diagnosis of various diseases.