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Formaline 7,5 %, buffered pH 7.0

Formalin 7.5%, Phosphate Buffer pH 7.0 is a ready-to-use solution widely used in histology and scientific laboratories. It consists of formaldehyde, di-sodium hydrogen phosphate dihydrate, sodium dihydrogen phosphate dihydrate and water. Formaldehyde (CH2O) is a strong aldehyde that serves as a fixative by cross-linking proteins and nucleic acids in tissue samples to preserve their structure. The phosphates (Na2HPO4 and NaH2PO4) serve as buffers to stabilize the pH of the solution at about 7.0, which is close to the physiological pH, allowing optimal preservation of tissue structures.

The solution is used as an in vitro diagnostic agent, particularly in histology, where it is used to fix tissue samples prior to further processing and analysis. Fixation stabilizes and preserves tissue structures, allowing detailed examination under the microscope.

The solution forms methylene bridges that cross-link proteins and nucleic acids, enabling effective fixation of cell structures. This product is suitable as a routine fixative in histology as well as scientific laboratory applications, providing consistent, reliable results. At the same time, the morphology of the tissues is preserved and their structural details are clearly visible.

The molarity of the formalin solution is approximately 2.5 mol/L, and that of the phosphate buffers is approximately 0.1 mol/L. The buffer capacity, which indicates how well the buffer can resist changes in pH, is about 0.15 mol/L.
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Article no.: 18727

Fixation of tissue samples

  •   Container size and order number Net price Amount
    Formaline 7,5 %, buffered pH 7.0 - 100 ml
    Order number: 18727.00100
    5,96 €
    Formaline 7,5 %, buffered pH 7.0 - 250 ml
    Order number: 18727.00250
    6,51 €
    Formaline 7,5 %, buffered pH 7.0 - 500 ml
    Order number: 18727.00500
    10,30 €
    Formaline 7,5 %, buffered pH 7.0 - 1.000 ml
    Order number: 18727.01000
    13,04 €
    Formaline 7,5 %, buffered pH 7.0 - 2.500 ml
    Order number: 18727.02500
    17,50 €
    Formaline 7,5 %, buffered pH 7.0 - 5.000 ml
    Order number: 18727.05000
    20,65 €
    Formaline 7,5 %, buffered pH 7.0 - 10.000 ml
    Order number: 18727.10000
    38,51 €
    Formaline 7,5 %, buffered pH 7.0 - 20.000 ml
    Order number: 18727.20000
    53,79 €
    Formaline 7,5 %, buffered pH 7.0 - 25.000 ml
    Order number: 18727.25000
    61,39 €

Important Information

UN-Nummer: 0000

Warning labels:

Lagerung: 15 … 25 °C

Haltbarkeit: 15 Monate

safety data sheet


product information

Relevant Incredients:
• Di-sodium hydrogen phosphate dihydrate
• Sodium di-hydrogen Phosphat 2-hydrate
• Formaldehyde ~37%, stabilised

instruction manual

Technical informations

specifications

Instructions / Protocol / Recommendations

Verwendung:

Bei der Fixierung werden alle in der Zelle ablaufenden Prozesse unterbrochen, unter möglichst optimaler Erhaltung des Zustandes und der Struktur des Gewebes. Biochemische Prozesse wie Autolyse, Verwesung und Fäulnis werden wirkungsvoll verhindert. Fixierlösung nach Stieve eignet sich zur Fixierung von Hodengewebe und Eierstockgewebe. Die Fixierlösung ist ausschließlich für die professionelle Anwendung als in-vitro Diagnostikum im histologischen Labor vorgesehen.

Hazard and safety instructions

Signal Word 1: Gefahr
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Warning labels: