This staining solution for fluorescence microscopy is a specially developed mixture containing ethidium bromide, acridine orange in aqueous solution. It serves as a critical component in numerous applications within the life sciences and medical diagnostics, particularly in cell biology and molecular biology.

The staining solution is characterized by its particular suitability for use in fluorescence microscopy protocols where specific cellular components, such as nucleic acids, need to be visualized. It allows clear and precise visualization of cellular and subcellular structures.

Chemically, the two fluorescent dyes, ethidium bromide and acridine orange, interact specifically with nucleic acids and produce a visible fluorescent signal when excited by light of specific wavelengths. Ethanol acts as a solvent and supports the permeability of the cell membrane, allowing the dyes to better penetrate the cell.

The functioning of the staining solution is based on these chemical interactions. When used, the fluorescent dyes bind to nucleic acids in the cell, allowing them to be visualized under a fluorescence microscope. This makes the staining solution an indispensable tool in life science research and diagnostics.