The staining kit LH: Trichrome staining according to PFEIFFER, WELLHÄUSER & GEHRA is primarily used for histology of tissue samples to distinguish mineralized bone particles from collagenous connective tissue and muscle tissue. Its application is particularly useful in food testing and medical diagnostics to check the quality of meat products and identify potential contamination by bone particles.

This kit is characterized by its special suitability for differentiating various tissue types. In particular, it provides valuable information when examining foods with high connective tissue content or unclear composition.

The kit consists of picric acid-alizarin red S solution, indigo carmine solution and acetic acid. Here, the picric acid-alizarin red S solution produces an orange-red staining of mineralized bone particles, while the indigo carmine solution stains collagenous connective tissue blue and muscle tissue green. Acetic acid is used to fix the colors.

The chemicals in the kit react specifically with the different tissue types to produce a colored image that can be analyzed under a microscope. These chemical reactions can effectively distinguish bone particles, collagenous connective tissue and muscle tissue from each other, providing accurate information on tissue composition.