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Staining Kit: Silverstaining after BODIAN

Staining kit: BODIAN silver plating is a reliable tool in in vitro diagnostics for selective visualization of neurofibrils. The embedded Protargol S component interacts specifically with neuronal structures, which are thus displayed in a distinct black on the microscopic sections. This is further enhanced by the specially formulated silver enhancer for BODIAN silver plating. Hydroquinone is used as a reducing agent to convert silver ions into elemental silver. Gold chloride and oxalic acid are used to remove the unbound silver ions while improving the clarity of the sections. Sodium thiosulfate is used to fix and stabilize the stain. The copper sheet is an important component of the kit as it serves as a catalyst in the silvering process. The resulting microscopic images are both high quality and high contrast, allowing effective analysis and examination.

Article no.: 16754

Neurofibril staining

  •   Container size and order number Net price Amount
    Staining Kit: Silverstaining after BODIAN - 100 ml
    Order number: 16754.00100
    208,43 €
    Staining Kit: Silverstaining after BODIAN - 250 ml
    Order number: 16754.00250
    480,22 €
    Staining Kit: Silverstaining after BODIAN - 500 ml
    Order number: 16754.00500
    782,79 €
    Staining Kit: Silverstaining after BODIAN - 1.000 ml
    Order number: 16754.01000
    1.515,85 €

Important Information

UN-Nummer: siehe Einzelprodukte

Warning labels:

Lagerung: siehe Einzelprodukte


product information

Components of this kit:
• Protargol S, Artikel-Nr.:00235
• Copper sheet, Artikel-Nr.:01756
• Hydroquinone 1 %, Artikel-Nr.:11143
• Goldchloride 0.1 %, Artikel-Nr.:11134
• Oxalic Acid 2 % , Artikel-Nr.:12704
• Sodium Thiosulfate 5 %, Artikel-Nr.:10288
• Kit: Silver Enhancer for BODIAN’s Silver Staining, Artikel-Nr.:16893

colouring protocol

Technical informations

specifications

Instructions / Protocol / Recommendations

Verwendung:

The BODIAN silver plating staining kit allows in vitro diagnosis of neurofibrils by silver impregnation for selective visualization of these structures in black. This staining process uses a specific composition of chemicals, such as Protargol S, copper sheet, hydroquinone, gold chloride, oxalic acid, sodium thiosulfate and silver enhancer. It is used in neuroscience and pathology to better study and diagnose neurological diseases and disorders.

Prinzip:

The staining kit for silvering according to BODIAN enables the detection of tissue or cell structures in in vitro diagnostics by a special color reaction. First, the Protargol S complex binds to specific proteins of the tissue or cell structure. Then, a chemical reaction is performed on the copper sheet using hydroquinone, gold chloride, oxalic acid and sodium thiosulfate. This reaction leads to the formation of silver particles, which allow visible silvering of the structures. By using the silver enhancer, the signal is intensified, allowing improved visualization and analysis of the target structures.

Verfahren:

The staining kit for silvering according to BODIAN enables the detection of tissue or cell structures in in vitro diagnostics by a special color reaction. First, the Protargol S complex binds to specific proteins of the tissue or cell structure. Then, a chemical reaction is performed on the copper sheet using hydroquinone, gold chloride, oxalic acid and sodium thiosulfate. This reaction leads to the formation of silver particles, which allow visible silvering of the structures. By using the silver enhancer, the signal is intensified, allowing improved visualization and analysis of the target structures.

Hazard and safety instructions

Signal Word 1: Gefahr
Signal Word 2: acid_red, Aquatic-pollut-red, exclam, silhouete
Warning labels: