Verwendung:

The Trichrome staining kit according to Wallart-Houette consists of different stock solutions and enables triple staining of tissue samples in the context of in vitro diagnostics. This method is used to visualize and distinguish between different tissue types and their components. Specifically, it is used to differentiate collagenous tissue (yellow), muscle tissue (red) and other components of connective and supporting tissue (reddish). This allows a wide range of histological issues to be addressed where differentiated color indicators are important.

Prinzip:

The Wallart-Houette Trichrome Staining Kit is a complex histological staining system for in vitro diagnosis of tissue or cellular structures. The components of the kit bind to various cellular components, resulting in differential staining. Acid fuchsin binds to more basic structures such as cell nuclei and muscle cells, while Echtgelb binds to more acidic tissues such as collagen or elastic fibers. Weigert stock solutions A and B allow selective detection of elastic fibers. The addition of acetic acid increases the binding selectivity of the dyes. The staining scheme allows differentiation of cell types and tissue structures for diagnostic purposes.

Verfahren:

The Wallart-Houette Trichrome Staining Kit is a complex histological staining system for in vitro diagnosis of tissue or cellular structures. The components of the kit bind to various cellular components, resulting in differential staining. Acid fuchsin binds to more basic structures such as cell nuclei and muscle cells, while Echtgelb binds to more acidic tissues such as collagen or elastic fibers. Weigert stock solutions A and B allow selective detection of elastic fibers. The addition of acetic acid increases the binding selectivity of the dyes. The staining scheme allows differentiation of cell types and tissue structures for diagnostic purposes.