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Staining Kit: Trichrome for elastic tissues

Trichrome staining kit for elastic fibers is widely used in histology, in vitro diagnostics and scientific laboratories. It consists of a mixture of picric acid sublimate, Verhöff staining solution, acid yellow, Biebrich solution, phosphotungstic acid, light green and acetic acid. The chemical formulas of these components are diverse and complex, with each playing a specific role in the staining reaction.

Picricric acid, a strong oxidizing agent, interacts with proteins and other biological materials to fix and preserve them. Verhöff staining solution, a mixture of hematoxylin and iron salts, stains elastic fibers black. Acid yellow, Biebrich solution and light green serve as contrast dyes that highlight different tissue types. Phosphotungstic acid and acetic acid are used as mordants and for pH regulation.

The chemical reactions that occur during dyeing are mainly ion exchange and complexation reactions. The dyes bind to specific components of the tissue, producing different colors that allow different structures to be distinguished under the microscope.

Article no.: 18490

Dyeing elastic fibers

  •   Container size and order number Net price Amount
    Staining Kit: Trichrome for elastic tissues - 100 ml
    Order number: 18490.00100
    156,59 €
    Staining Kit: Trichrome for elastic tissues - 250 ml
    Order number: 18490.00250
    289,61 €
    Staining Kit: Trichrome for elastic tissues - 500 ml
    Order number: 18490.00500
    586,19 €
    Staining Kit: Trichrome for elastic tissues - 1.000 ml
    Order number: 18490.01000
    1.135,33 €
    Staining Kit: Trichrome for elastic tissues - 2.500 ml
    Order number: 18490.02500
    2.678,43 €

Important Information

UN-Nummer: siehe Einzelprodukte

Warning labels:

Lagerung: siehe Einzelprodukte


product information

Components of this kit:
• Picric acid sublimate solution, Artikel-Nr.:18480
• Kit: VERHOEFF’s Staining Solution, Artikel-Nr.:10402
• Acid Yellow 1 % , Artikel-Nr.:18485
• Biebrich’s solution, Artikel-Nr.:18475
• Phosphortungstic Acid 2 %, Artikel-Nr.:10321
• Light Green 2 %, Artikel-Nr.:11270
• Acetic Acid 5 %, Artikel-Nr.:11727

colouring protocol

instruction manual

Technical informations

specifications

Instructions / Protocol / Recommendations

Verwendung:

Bei dem Färbekit Masson Goldner Trichrom handelt es sich um eine Trichromfärbung zur differenzierten Bindegewebsdarstellung. Das Bindegewebe wird in kontrastierenden Farben zur Umgebung (Cytoplasma, Muskelfasern) angefärbt. Zur Färbung werden Gefrierschnitte, Dünnschicht-Präparate, und Paraffinschnitte verwendet. Die Färbelösung ist ausschließlich für die professionelle Anwendung als in-vitro Diagnostikum im histologischen Labor vorgesehen.

Hazard and safety instructions

Signal Word 2: flamme, exclam, ,
Warning labels: