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Staining Kit: TB Staining (cold) after ZIEHL-NEELSEN

The Ziehl-Neelsen TB staining kit (cold) is a specially developed staining kit for the detection of acid-fast mycobacteria such as Mycobacterium tuberculosis. Ziehl-Neelsen cold staining is an alternative method to Ziehl-Neelsen hot staining in the diagnosis of tuberculosis and other infections caused by acid-fast bacteria. Cold staining does not require heating of the specimen and is therefore advantageous in situations where a heat source is not available or heating is undesirable.

The staining kit contains the following components:

1. MucoFlutol (Item No.:12097): This solution is used to pre-treat the specimen to ensure optimal staining.
2. Ziehl-Neelsen carbol fuchsin (cold stain, Item No.:13070): This red dye allows good penetration into the cells. The sample is first treated with the cold fuchsin solution, with dye binding to acid-fast bacteria without heating.
3. Hydrochloric acid alcohol (3 % / 90 %, Item No.:12255): After treatment with cold fuchsin, the sample is treated with a hydrochloric acid alcohol solution to decolorize non-acid-fast cells and structures. Acid-fast bacteria retain the cold fuchsin dye and appear bright red in color, while other cell structures lose color.
4. Malachite Green Oxalate Solution (Item No.:12249): To improve visualization and differentiation of structures within the tissue sample, counterstain with malachite green oxalate. This solution stains the background and non-acid-fast cells a green hue, making the acid-fast bacteria easier to identify in contrast to other cellular structures.

The Ziehl-Neelsen TB Staining Kit (cold) allows laboratories to achieve reliable and accurate staining of tissue samples, which is essential for the study of Mycobacterium tuberculosis and other acid-fast bacteria. The kit offers an optimal combination of reagents that ensure easy and efficient performance of staining to achieve meaningful results for diagnostics and research.

Article no.: 12243

Tuberculosis detection

  •   Container size and order number Net price Amount
    Staining Kit: TB Staining (cold) after ZIEHL-NEELSEN - 100 ml
    Order number: 12243.00100
    29,84 €
    Staining Kit: TB Staining (cold) after ZIEHL-NEELSEN - 250 ml
    Order number: 12243.00250
    45,48 €
    Staining Kit: TB Staining (cold) after ZIEHL-NEELSEN - 500 ml
    Order number: 12243.00500
    84,79 €
    Staining Kit: TB Staining (cold) after ZIEHL-NEELSEN - 1.000 ml
    Order number: 12243.01000
    163,19 €
    Staining Kit: TB Staining (cold) after ZIEHL-NEELSEN - 2.500 ml
    Order number: 12243.02500
    367,59 €

Important Information

UN-Nummer: siehe Einzelprodukte

Warning labels:

Lagerung: siehe Einzelprodukte


product information

Components of this kit:
• MucoFlutol, Artikel-Nr.:12097
• Carbol-Fuchsin after ZIEHL-NEELSEN (cold Staining), Artikel-Nr.:13070
• Hydrochloric Acid in Ethanol (3 % / 90 %), Artikel-Nr.:12255
• Malachite Green-Oxalate, Artikel-Nr.:12249

colouring protocol

instruction manual

Technical informations

specifications

Hazard and safety instructions

Signal Word 1: Gefahr
Signal Word 2: silhouete, acid_red, flamme, exclam
Warning labels: