Staining Kit: SHOOBRIDGE’s Polychrome Staining
The application of the staining kit involves several steps. First, ammonium iron(III) sulfate, hematoxylin according to LILLIE and hydrochloric acid alcohol are used in the staining process to initiate the chemical process of staining. Then naphthol yellow solution, phosphotungstic acid orange G, phosphotungstic acid acid fuchsin and phosphotungstic acid methylene blue are used. These solutions allow specific reactions and interactions between the chemical components and the tissue structures, resulting in different color shades and brightness.
The complex chemical and physical processes during staining allow identification and differentiation of different tissue structures. The obtained staining results can contribute to the detailed analysis and study of the investigated structures.
The SHOOBRIGDE polychrome staining kit contains the necessary chemical solutions to achieve optimal staining results without the need to consider quantity specifications of the ingredients. The factual and technically precise wording of the product description is aimed at a scientific audience and avoids superficial advertising phrases or repetition of content. If the instructions are followed carefully, meaningful and differentiated staining results can be achieved with the SHOOBRIGDE polychrome staining kit.
Article no.: 15786
Staining of tissue samples
Important Information
UN-Nummer: siehe Einzelprodukte
Lagerung: siehe Einzelprodukte
product information
Components of this kit:
• Ammonium Iron (III) Sulfate with Glycerine, Artikel-Nr.:15535
• Hematoxylin after LILLIE, Artikel-Nr.:15541
• Hydrochloric acid Alcohol (1 % / 70 %), Artikel-Nr.:10372
• Naphthol Yellow 1 %, Artikel-Nr.:15547
• Phosphortungstic Acid - Orange G (C), Artikel-Nr.:15768
• Phosphortungstic Acid - Acid Fuchsine, Artikel-Nr.:15774
• Phosphortungstic Acid - Methylene Blue, Artikel-Nr.:15780
Instructions / Protocol / Recommendations
Verwendung:
The SHOOBRIDGE polychrome staining kit is used for staining tissue samples in in vitro diagnostics. This method is used for differential staining of collagen and other tissue structures in microscopic specimens. The use of ammonium iron (III) sulfate, hematoxylin according to LILLIE, hydrochloric acid alcohol, naphthol yellow solution, phosphotungstic acid - orange G, phosphotungstic acid - acid fuchsin and phosphotungstic acid - methylene blue produces a stain that makes it possible to distinguish different cell structures and components of the tissue and thus perform detailed histological analyses.
Prinzip:
The functional principle of SHOOBRIGDE polychrome staining lies in the selective binding of the various components to specific tissue or cell structures. Ammonium iron(III) sulfate and hematoxylin serve as oxidant and dye, binding to cell nuclei and chromatin. Hydrochloric acid alcohol fixes and removes excess dye, while naphthol yellow solution 1% helps to stain collagen. Phosphotungstic acids form complexes with various dyes (orange G, acid fuchsin, and methylene blue) that bind to different cell structures and components, providing a differentiated and detailed picture of the samples. This enables more accurate analysis in in vitro diagnostics.
Verfahren:
The functional principle of SHOOBRIGDE polychrome staining lies in the selective binding of the various components to specific tissue or cell structures. Ammonium iron(III) sulfate and hematoxylin serve as oxidant and dye, binding to cell nuclei and chromatin. Hydrochloric acid alcohol fixes and removes excess dye, while naphthol yellow solution 1% helps to stain collagen. Phosphotungstic acids form complexes with various dyes (orange G, acid fuchsin, and methylene blue) that bind to different cell structures and components, providing a differentiated and detailed picture of the samples. This enables more accurate analysis in in vitro diagnostics.