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Staining Kit: NISSL’s Staining

The NISSL staining kit is a special staining kit used in histology and neuroscience to visualize neurons and their components. NISSL staining allows visualization of neurons and their nuclei, providing important information about cell morphology, distribution, and organization of neurons in different areas of the nervous system.

The main component of the NISSL staining kit is cresylecht violet, a basic dye that binds to acidic structures such as ribonucleic acids (RNA) in the nuclei and rough endoplasmic reticulum of neurons. This stains the cell nuclei in a blue to purple hue, while the rough endoplasmic reticulum, called Nissl substance, is also visualized.

NISSL staining is particularly useful for studying the distribution and morphology of neurons in different brain regions because it provides high resolution and contrast between different cellular structures. By using NISSL staining, researchers and clinicians can obtain detailed information about the structure and organization of neural tissues and identify pathological changes in neurodegenerative diseases, injuries, or infections.

Overall, the NISSL staining kit provides an efficient and reliable method for studying neurons and their components, which is of great importance for the study of the nervous system and the diagnosis of neurological diseases.

Article no.: 12159

Staining of tissue samples

  •   Container size and order number Net price Amount
    Staining Kit: NISSL’s Staining - 100 ml
    Order number: 12159.00100
    60,85 €
    Staining Kit: NISSL’s Staining - 250 ml
    Order number: 12159.00250
    82,86 €
    Staining Kit: NISSL’s Staining - 500 ml
    Order number: 12159.00500
    164,08 €
    Staining Kit: NISSL’s Staining - 1.000 ml
    Order number: 12159.01000
    313,22 €

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product information

Components of this kit:
• Potassium Metabisulfite 2 %, Artikel-Nr.:11149
• Cresyl Fast Violet for NISSL, Artikel-Nr.:11128
• Sodium Acetate Buffer, Artikel-Nr.:12142

colouring protocol

Technical informations

specifications

Instructions / Protocol / Recommendations

Verwendung:

The NISSL staining kit is used to stain tissue samples in histology and neuroscience to visualize neurons and their components, including nuclei and Nissl substance. It enables the study of cell morphology, distribution and organization of neurons in the nervous system. This is critical for the study of nervous tissue, identification of pathological changes in neurodegenerative diseases, injuries or infections, and diagnosis of neurological diseases.

Prinzip:

NISSL staining is a histological staining method for visualizing neurons and their structures in tissue sections. In this process, the dye cresylecht violet binds selectively to ribonucleic acids (RNA) in the rough endoplasmic reticulum and Nissl substances found in the cell bodies of nerve cells. Potassium metabisulfite and sodium acetate buffer are used as solvents and for pH regulation to ensure optimal staining. This method allows visualization of cell bodies and dendrites, enabling analysis of differences in neuronal cell morphology and organization in in vitro diagnostics.

Verfahren:

NISSL staining is a histological staining method for visualizing neurons and their structures in tissue sections. In this process, the dye cresylecht violet binds selectively to ribonucleic acids (RNA) in the rough endoplasmic reticulum and Nissl substances found in the cell bodies of nerve cells. Potassium metabisulfite and sodium acetate buffer are used as solvents and for pH regulation to ensure optimal staining. This method allows visualization of cell bodies and dendrites, enabling analysis of differences in neuronal cell morphology and organization in in vitro diagnostics.

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