Staining Kit: NEISSER’s Staining (Diphtheria Detection)
The special suitability of this kit lies in the Neisser stain, which allows differentiation of morphological characteristics and granules in Corynebacterium diphtheriae. The staining assists in the identification and diagnosis of diphtheria infections.
The chemical mode of operation is based on a sequential application of the three NEISSER solutions, first using methylene blue as a counterstain, followed by crystal violet and chrysoidin. This combination of dyes provides a high-contrast visualization of the bacteria under investigation, facilitating the identification of morphological features.
Compared to other staining methods, the NEISSER staining kit (diphtheria detection) is characterized by its high specificity and sensitivity in the identification of Corynebacterium diphtheriae. By combining the three NEISSER solutions, optimal staining is achieved, allowing reliable diagnosis and differentiation of diphtheria-causing bacteria.
Article no.: 13286
Detection of diphtheria bacteria
Important Information
Sales restriction: no private users!
UN-Nummer: siehe Einzelprodukte
Lagerung: siehe Einzelprodukte
product information
Components of this kit:
• NEISSER’s Solution I (Methylene Blue), Artikel-Nr.:13274
• NEISSER’s Solution II (Crystal Violet), Artikel-Nr.:13278
• NEISSER’s Solution III (Chrysoidine), Artikel-Nr.:13282
Instructions / Protocol / Recommendations
Verwendung:
The NEISSER staining kit (diphtheria detection) is an in vitro diagnostic tool used in microbiology and bacteriology for the identification and diagnosis of diphtheria infections. Using a combination of methylene blue, crystal violet, and chrysoidin, Neisser staining allows the differentiation of morphological characteristics and granules in Corynebacterium diphtheriae, the Gram-positive, pleomorphic rod bacterium that causes diphtheria. Sequential application of these three solutions results in high-contrast, specific, and sensitive visualization of the bacteria under study and aids in the identification of diphtheria infections.
Prinzip:
The NEISSER staining kit is used for the identification of diphtheria pathogens (Corynebacterium diphtheriae) in in vitro diagnostics. The principle is based on the selective staining of specific bacterial structures by the various dyes. Methylene blue stains predominantly the metachromatic granules, while crystal violet stains the cell walls of the bacteria. Chrysoidin is used for background staining to increase the contrast between bacteria and their surroundings during microscopic analysis. Due to the specific binding of the dyes to different structures, clear and effective visualization of diphtheria pathogens is possible.
Verfahren:
The NEISSER staining kit is used for the identification of diphtheria pathogens (Corynebacterium diphtheriae) in in vitro diagnostics. The principle is based on the selective staining of specific bacterial structures by the various dyes. Methylene blue stains predominantly the metachromatic granules, while crystal violet stains the cell walls of the bacteria. Chrysoidin is used for background staining to increase the contrast between bacteria and their surroundings during microscopic analysis. Due to the specific binding of the dyes to different structures, clear and effective visualization of diphtheria pathogens is possible.