The Rhodanine Copper Detection Staining Kit serves as a specialized tool in medical diagnostics and histology for identifying copper deposits in tissue samples. It is shown to be useful in the study of disorders of copper metabolism, such as Wilson's disease.

The kit's special suitability lies in its high sensitivity and specificity for copper, resulting in accurate and reliable results. The use of hematoxylin as a counterstain allows detailed morphological evaluation of the tissues examined.

The rhodanine solution reacts specifically with copper ions to form a red dye, with hematoxylin serving as a blue-violet counterstain. This combination allows simultaneous visualization of copper distribution and overall tissue structure.

In the application, tissue samples are first treated with the rhodanine solution, where a chemical reaction between rhodanine and the copper ions results in visible red staining. Counterstaining with hematoxylin is then performed to provide histological context. Together, this staining kit provides a reliable method for detecting copper in tissue samples.