Verwendung:

The Iron HALE staining kit is used for in vitro diagnostics and allows precise examination and identification of acidic mucosubstances present in renal cell carcinomas. It uses colloidal iron ions that react with carboxyl and sulfate residues to stain muco substances blue. A Berlin blue reaction can then be used to differentiate chromophobe from light cell renal cell carcinoma. This method offers high sensitivity and selectivity, which contributes to accurate diagnoses and optimized treatment decisions. It is also easy to use and enables reproducible results in medical diagnostics and biomedical research.

Prinzip:

The functional principle of iron HALE staining is based on the binding of colloidal iron to tissue or cell structures. The acetic acid enables better binding of the iron, while potassium ferricyanide (II) leads to the formation of berliner blue, which serves as a visible dye. The hydrochloric acid stabilizes the reaction environment and neutral red serves as a contrast dye. This interaction of components allows selective staining of structures, such as cell nuclei or fibers, for easier visual examination and diagnostics in the laboratory during in vitro experiments.

Verfahren:

The functional principle of iron HALE staining is based on the binding of colloidal iron to tissue or cell structures. The acetic acid enables better binding of the iron, while potassium ferricyanide (II) leads to the formation of berliner blue, which serves as a visible dye. The hydrochloric acid stabilizes the reaction environment and neutral red serves as a contrast dye. This interaction of components allows selective staining of structures, such as cell nuclei or fibers, for easier visual examination and diagnostics in the laboratory during in vitro experiments.