Staining Kit: Prussian Blue [Iron(III) Detection]
This method, also known as Perl's Prussian Blue or Turnbull's Blue, is based on the reaction of iron salts with a potassium hexacyanoferrate(III) solution to produce the characteristic Berlinerblau pigment. The staining kit is ideal for testing tissue samples that contain iron deposits, such as those found in hemochromatosis, hemosiderosis, or other diseases associated with iron overload.
The Berlinerblau staining kit contains potassium ferricyanide(III) solution that reacts with the ferric ions in the tissue sample to produce the Berlinerblau pigment. This pigment is blue and identifies iron deposits in the tissue sample. The stain is specific for ferric ions and provides high sensitivity and selectivity in the detection of iron deposits.
For better visualization and differentiation of cellular and extracellular structures within the tissue sample, a counterstain, such as with hematoxylin, can be used. Hematoxylin stains cell nuclei a blue hue that provides a useful contrast to the berliner blue pigment.
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Article no.: 11097
Iron detection
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product information
Components of this kit:
• Potassium Ferrocyanide (II) 5 % (Yellow Prussiate), Artikel-Nr.:11333
• Hydrochloric Acid 5 % for Iron Detection, Artikel-Nr.:11632
• Seed red 0,1 %, Artikel-Nr.:10264
Instructions / Protocol / Recommendations
Verwendung:
The Berlin Blue Reaction is a histochemical method for the detection of trivalent iron in tissue preparations. It can still detect 0.002 µg of iron in tissue. Frozen sections, thin section preparations, and kerosene sections are used for staining. The staining solution is intended exclusively for professional use as an in vitro diagnostic agent in the histology laboratory.
Prinzip:
In the Berlin blue reaction, ionic iron (Fe3+) not bound to the heme scaffold reacts with potassium ferricyanide(II) in hydrochloric acid solution. It precipitates as an insoluble complex salt in the tissue cells and free cellular iron is visualized. The tissue preparation is counterstained with nuclear red. The cytoplasm turns pale pink. This achieves optimal differentiation of the iron deposits in the tissue.
Verfahren:
In the Berlin blue reaction, ionic iron (Fe3+) not bound to the heme scaffold reacts with potassium ferricyanide(II) in hydrochloric acid solution. It precipitates as an insoluble complex salt in the tissue cells and free cellular iron is visualized. The tissue preparation is counterstained with nuclear red. The cytoplasm turns pale pink. This achieves optimal differentiation of the iron deposits in the tissue.
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