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Staining Kit: Aldehydfuchsin Triple Staining

Aldehyde fuchsin triple staining is a three-component stain used to visualize elastic fibers (dark red) with differentiation of collagenous connective tissue and cytoplasm (yellow), as well as mucins (reddish-purple) and cell nuclei (blue-green). This method combines three dyes - paraldehyde fuchsin, pontacyl blue-black and true yellow - to selectively stain different structures within tissue samples.

Paraldehyde fuchsin is used to prepare the aldehyde fuchsin working solution and stains elastic fibers an intense dark red. Elastic fibers play an important role in maintaining the structure and elasticity of tissues. Likewise, paraldehyde fuchsin stains mast cell granules, which have a significant role in inflammatory and allergic reactions, purple.

Pontacyl blue black is a dye responsible for staining collagen fibers and reticular fibers. Collagen fibers appear blue and are the main component of connective tissue, which provides the support structure for many organs and tissues in the body. Reticular fibers are also colored blue and form a fine network that provides structural support for cells and tissues, especially in lymphoid organs.
Real yellow is a dye that stains cell nuclei and cytoplasm a yellowish-brown. The stain helps distinguish cell nuclei from other cellular structures and allows assessment of cell morphology and architecture.

Aldehyde fuchsin triple staining offers the advantage of distinguishing and visualizing different cellular and extracellular structures within a single staining. This method is particularly suitable for the analysis of tissue samples where elastic fibers, mast cell granules, collagen fibers, reticular fibers, mucins and cell nuclei are of interest.

Article no.: 11086

Staining of tissue samples

Important Information

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product information

Components of this kit:
• Paraldehyde-Fuchsin (Stock Solution), Artikel-Nr.:12763
• Acid Black Solution, Artikel-Nr.:10348
• Fast Yellow 2 %, alcoholic, Artikel-Nr.:10168

Technical informations

specifications

Instructions / Protocol / Recommendations

Verwendung:

The Aldehyde Fuchsin Triple Staining Kit is designed for in vitro diagnostics of tissue samples. It is used for selective staining of elastic fibers (dark red), collagenous connective tissue and cytoplasm (yellow), as well as mucins (reddish-purple) and cell nuclei (blue-green). The combination of paraldehyde fuchsin, pontacyl blue-black and true yellow allows differentiation and visualization of various cellular and extracellular structures, especially in specimens where elastic fibers, mast cell granules, collagen fibers, reticular fibers and cell nuclei are important.

Prinzip:

The functional principle of aldehyde fuchsin triple staining is based on the binding of the color components to different tissue or cell structures. Aldehyde fuchsin binds to acidic structures such as mast cell granules and elastic fibers, pontacyl blue black binds to basic structures such as collagen fibers, and true yellow binds to neutral structures such as cell nuclei. These bindings enable differentiated visualization of tissue and cell structures and facilitate in vitro diagnostics when examining histological samples.

Verfahren:

The functional principle of aldehyde fuchsin triple staining is based on the binding of the color components to different tissue or cell structures. Aldehyde fuchsin binds to acidic structures such as mast cell granules and elastic fibers, pontacyl blue black binds to basic structures such as collagen fibers, and true yellow binds to neutral structures such as cell nuclei. These bindings enable differentiated visualization of tissue and cell structures and facilitate in vitro diagnostics when examining histological samples.

Hazard and safety instructions

Signal Word 2: flamme, skull, ,
Warning labels: