Herzlich Willkommen bei Morphisto!

Wir bitten unsere österreichischen Kunden, den Shop unseres Partners Sanova GesmbH zu besuchen.

  Contact form
(+49) 069/4003019-60
€ 0.00
Product Qty Price
Plus shipping
Total € 0.00

Staining kit: Alcian blue-core red for acidic mucosubstances

Alcian blue nuclear red staining kit is used in histology and histochemistry to visualize acid mucosubstances in tissue sections. The main application is in the study of acid mucosubstances found in various tissues such as glands, cartilage and connective tissue.

The special suitability of this kit lies in the combined staining of acid mucosubstances with Alcian blue and a complementary nuclear staining with nuclear red. Alcian blue selectively binds to acidic polysaccharides and stains them blue, while nuclear red causes a red-orange staining of cell nuclei, facilitating differentiation of structures.

The chemical mode of operation is based on the interaction of Alcian blue with acidic polysaccharides through electrostatic attraction. The acidic pH environment is generated by acetic acid, which allows Alcian blue to bind optimally to acidic mucosubstances. Nuclear red is a basic dye that binds to DNA and thus highlights cell nuclei.

Article no.: 13416

Detection of mucopolysaccharides

  •   Container size and order number Net price Amount
    Staining kit: Alcian blue-core red for acidic mucosubstances - 100 ml
    Order number: 13416.00100
    57,70 €
    Staining kit: Alcian blue-core red for acidic mucosubstances - 250 ml
    Order number: 13416.00250
    86,05 €
    Staining kit: Alcian blue-core red for acidic mucosubstances - 500 ml
    Order number: 13416.00500
    169,79 €
    Staining kit: Alcian blue-core red for acidic mucosubstances - 1.000 ml
    Order number: 13416.01000
    325,79 €
    Staining kit: Alcian blue-core red for acidic mucosubstances - 2.500 ml
    Order number: 13416.02500
    746,71 €

Important Information

UN-Nummer: siehe Einzelprodukte

Lagerung: siehe Einzelprodukte


product information

Components of this kit:
• Alcian blue 1 % (pH 2,5 in acetic acid), Artikel-Nr.:12696
• Acetic Acid 3 %, Artikel-Nr.:11384
• Seed red 0,1 %, Artikel-Nr.:10264

colouring protocol

Technical informations

specifications

Instructions / Protocol / Recommendations

Verwendung:

Alcianblau wird in der Histologie verwendet, um sauro Mukosubstanzen (saure Mucine und sulfatierte Glykosaminoglykane wie Hyaluronsäure oder Chondroitinsulfat) bei niedrigem pH-Wert (1,0 bis 2,5) selektiv anzufärben. Je nach pH-Wert der Färbelösung können spezifische Mucine nachgewiesen werden. Eine weitere Methode der selektiven Darstellung, ist die Variation der Elektrolytkonzentration. Mit steigender Ionenstärke wird eine Färbung mit Alcianblau verhindert, wodurch eine Differenzierung unterschiedlich stark sulfatierter Mucopolysaccharide sowie Heparine, Heparinsulfate und Keratinsulfate möglich ist.

Hazard and safety instructions