Erylysis-Buffer pH 7.2 - 7.4 (sterilized)
The Erylysis Buffer pH 7.2 - 7.4 (sterile filtered) is a specialized laboratory chemical mainly used for lysis of erythrocytes in blood samples. The buffer consists of ammonium chloride, potassium hydrogen carbonate and EDTA disodium salt dihydrate dissolved in ultrapure water and sterile filtered through a 0.2 µm attachment filter.
When used in laboratory practice, the buffer is applied to blood samples, with the ammonium chloride creating hypotonic conditions under which red blood cells are lysed. At the same time, the potassium bicarbonate stabilizes pH and the EDTA disodium salt acts as a chelator to bind divalent cations that might otherwise interfere with lysis.
Application of this buffer results in effective lysis of red cells and release of intracellular components, allowing subsequent analysis of leukocytes and other blood components. The buffer provides a reliable and reproducible method for blood cell lysis in various research and clinical applications.
When used in laboratory practice, the buffer is applied to blood samples, with the ammonium chloride creating hypotonic conditions under which red blood cells are lysed. At the same time, the potassium bicarbonate stabilizes pH and the EDTA disodium salt acts as a chelator to bind divalent cations that might otherwise interfere with lysis.
Application of this buffer results in effective lysis of red cells and release of intracellular components, allowing subsequent analysis of leukocytes and other blood components. The buffer provides a reliable and reproducible method for blood cell lysis in various research and clinical applications.
Article no.: 15038
Preparation of buffer solutions
product information
Relevant Incredients:
• ammonium chloride
• Potassium hydrogen carbonate
• EDTA