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Eosin 0,5 %, alcoholic 90 %, acid

Eosin 0.5%, alcoholic 90%, acidified is a solution containing the synthetic dye eosin (tetrabromofluorescein, molecular formula: C_20H_8Br_4O_5) in a 90% alcohol solution with a concentration of 0.5% and additionally acidified with an acid, such as acetic acid. This acidified eosin solution is mainly used in histology and cytology to stain cell structures and the extracellular matrix. Eosin has a high affinity for basic structures such as proteins and typically stains them pink or red.

Acidifying the eosin solution with acetic acid affects the staining kinetics and quality. Acetic acid is a weak acid that lowers the pH of the solution. The decrease in pH increases the binding affinity of eosin to basic amino acids such as lysine and arginine in proteins. This results in sharper and more differentiated staining. In addition, acetic acid accelerates the staining process by promoting the penetration of the dye into the tissue.

The 0.5% alcoholic acidified eosin solution is thus a powerful option for histological and cytological applications. The higher concentration of eosin and acidification with acetic acid results in improved staining kinetics, increasing the color intensity and sharpness of the stain and allowing better differentiation of cellular and extracellular structures.

Article no.: 19127

Staining of tissue samples

  •   Container size and order number Net price Amount
    Eosin 0,5 %, alcoholic 90 %, acid - 100 ml
    Order number: 19127.00100
    14,51 €
    Eosin 0,5 %, alcoholic 90 %, acid - 250 ml
    Order number: 19127.00250
    17,99 €
    Eosin 0,5 %, alcoholic 90 %, acid - 500 ml
    Order number: 19127.00500
    34,65 €
    Eosin 0,5 %, alcoholic 90 %, acid - 1.000 ml
    Order number: 19127.01000
    34,48 €
    Eosin 0,5 %, alcoholic 90 %, acid - 2.500 ml
    Order number: 19127.02500
    65,03 €

Important Information

UN-Nummer: 1170

Warning labels:

Lagerung: 15 … 25 °C

Haltbarkeit: 24 Monate

safety data sheet


product information

Relevant Incredients:
• Ethyl alcohol
• Eosin Y (C.I.: 45380)
• Acetic acid 99%
• 1-Propanol

instruction manual

Technical informations

specifications

Instructions / Protocol / Recommendations

Verwendung:

Eosinfärbelösung dient nach einer Kernfärbung mit Hämatoxilin zur Gegenfärbung von Proteinen, Bindegewebe, Fasern und Keratin. Zur Färbung werden Abstrichpräparate, Gefrierschnitte, Dünnschicht-präparate, oder Paraffinschnitte verwendet. Die Färbelösung ist ausschließlich für die professionelle Anwendung als in-vitro Diagnostikum im histologischen bzw. zytologischen Labor vorgesehen. Die Färbelösung Eosin 1%ig, alkoholisch kann einzeln oder in Kombination mit Hämatoxylin (z. B. Artikel-Nr.: 10231) in der H&E-Färbung angewendet werden.

Hazard and safety instructions

Signal Word 1: Gefahr
Signal Word 2: flamme, acid_red, ,
Warning labels: