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GRAM’s Decolorizing Solution

Gram's decolorizing solution is an important component of Gram's stain, a classical microbiological staining method developed to classify bacteria into two main groups: Gram-positive and Gram-negative. The distinction is based on differences in the cell wall structure of the bacteria.

The destaining solution typically consists of a mixture of isopropanol and acetone. Destaining is a critical step in Gram staining because it removes the dyes from the Gram-negative bacteria while the Gram-positive bacteria retain their staining.
The Gram staining process consists of several steps:

1. Fixation of the specimen to the slide.
2. Staining with crystal violet solution, which stains both Gram-positive and Gram-negative bacteria.
3. Treatment with Lugol's solution (iodine solution), which acts as a mordant and enhances dye binding in bacterial cells.
4. Destaining with Gram's destaining solution. Gram-positive bacteria retain their purple color, while Gram-negative bacteria are decolorized.
5. Counterstain with a red dye solution, such as safranin. The decolorized Gram-negative bacteria take on this color and appear red, while the Gram-positive bacteria remain purple.

Article no.: 11499

Post-treatment of Gram stains

  •   Container size and order number Net price Amount
    GRAM’s Decolorizing Solution - 100 ml
    Order number: 11499.00100
    10,58 €
    GRAM’s Decolorizing Solution - 250 ml
    Order number: 11499.00250
    14,99 €
    GRAM’s Decolorizing Solution - 500 ml
    Order number: 11499.00500
    19,60 €
    GRAM’s Decolorizing Solution - 1.000 ml
    Order number: 11499.01000
    21,69 €
    GRAM’s Decolorizing Solution - 2.500 ml
    Order number: 11499.02500
    37,30 €

Important Information

UN-Nummer: 1219

Warning labels:

Lagerung: 15 … 25 °C

Haltbarkeit: 48 Monate

safety data sheet

product information

Relevant Incredients:
• Isopropyl alcohol
• Acetone

Technical informations


Hazard and safety instructions

Signal Word 1: Gefahr
Signal Word 2: flamme, exclam, ,
Warning labels:

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