EDTA-Buffer 0.1 mol/l, pH 8.0
EDTA buffer 0.1 mol/l, pH 8.0 is mainly used in biological and biochemical research fields as well as in quality control of food and environmental samples. One of the main uses is the stabilization of enzymes and proteins, as this buffer has the ability to bind metal ions that could cause potential interference in sensitive biochemical reactions.
The chemical mode of operation is based on the presence of ethylenediaminetetraacetic acid (EDTA), a chelating compound that can bind metal ions such as calcium, magnesium and iron. By forming stable EDTA-metal complexes, these metal ions are removed from the reaction mixture and inactivated, stabilizing enzymatic activity and protein structure. The pH adjustment of the buffer to 8.0 enables optimal chelation and helps to ensure that biological processes can proceed in a suitable pH range.
The special suitability of EDTA Buffer 0.1 mol/l, pH 8.0 results from its effectiveness in preventing metal ion-related interference in biological systems and reducing non-specific binding. For example, DNA extractions, enzyme assays and protein crystallizations can provide accurate and reliable results in the presence of this buffer. The aqueous base of the buffer ensures good solubility and miscibility with most biological samples and reagents, while the EDTA concentration has been carefully selected to 0.1 mol/l to ensure effective chelation.
The chemical mode of operation is based on the presence of ethylenediaminetetraacetic acid (EDTA), a chelating compound that can bind metal ions such as calcium, magnesium and iron. By forming stable EDTA-metal complexes, these metal ions are removed from the reaction mixture and inactivated, stabilizing enzymatic activity and protein structure. The pH adjustment of the buffer to 8.0 enables optimal chelation and helps to ensure that biological processes can proceed in a suitable pH range.
The special suitability of EDTA Buffer 0.1 mol/l, pH 8.0 results from its effectiveness in preventing metal ion-related interference in biological systems and reducing non-specific binding. For example, DNA extractions, enzyme assays and protein crystallizations can provide accurate and reliable results in the presence of this buffer. The aqueous base of the buffer ensures good solubility and miscibility with most biological samples and reagents, while the EDTA concentration has been carefully selected to 0.1 mol/l to ensure effective chelation.
Article no.: 12986
Preparation of buffer solutions
Important Information
Lagerung: 4 … 8 °C
Haltbarkeit: 12 Monate
product information
Relevant Incredients:
• Ethylenediaminetetraacetic acid