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EDTA Decalcifying Solution ~ 20 %, pH 7.0 - 7.2

EDTA decalcification solution, with approximately 20% concentration and a pH of 7.0 to 7.2, is used in histology and pathology to prepare bone and calcified tissue specimens for microscopic examination. EDTA (ethylenediaminetetraacetic acid) is a chelating agent that selectively removes calcium ions from tissue, thereby dissolving calcifications.

The 20% EDTA decalcifying solution is prepared by dissolving EDTA in water and then adjusting to a pH of approximately 7.0 to 7.2. The pH of 7.0 - 7.2 is close to the physiological pH, which can help to maintain the morphology and structure of the tissue during the decalcification process. The higher concentration allows accelerated decalcification of tissue specimens by extracting the calcium ions from the tissue without affecting the morphological and cellular structures.

Because the concentration of EDTA solution is higher than that of the 10% or 13% solution, decalcification can be performed in a shorter time. However, this depends on the size and density of the sample and may still take several days. The solution should be changed regularly to ensure efficient decalcification.

The 20% EDTA decalcification solution, with a pH of 7.0 to 7.2, is specifically designed for professional use in histology and pathology laboratories and provides an effective and gentle method for preparing bone and calcified tissue specimens for microscopic examination. The solution is also suitable for decalcification of samples to be prepared for immunohistochemistry or in situ hybridization, as it largely preserves antigenicity and nucleic acids.

Article no.: 16529

Decalcification of tissue samples

  •   Container size and order number Net price Amount
    EDTA Decalcifying Solution ~ 20 %, pH 7.0 - 7.2 - 100 ml
    Order number: 16529.00100
    23,48 €
    EDTA Decalcifying Solution ~ 20 %, pH 7.0 - 7.2 - 250 ml
    Order number: 16529.00250
    39,33 €
    EDTA Decalcifying Solution ~ 20 %, pH 7.0 - 7.2 - 500 ml
    Order number: 16529.00500
    57,42 €
    EDTA Decalcifying Solution ~ 20 %, pH 7.0 - 7.2 - 1.000 ml
    Order number: 16529.01000
    94,60 €
    EDTA Decalcifying Solution ~ 20 %, pH 7.0 - 7.2 - 2.500 ml
    Order number: 16529.02500
    207,59 €

Important Information

UN-Nummer: 0000

Warning labels:

Lagerung: 15 … 25 °C

Haltbarkeit: 12 Monate

safety data sheet

product information

Relevant Incredients:
• EDTA
• Sodium hydroxide

Technical informations

specifications

Hazard and safety instructions

Signal Word 1: Achtung
Signal Word 2: exclam, silhouete, ,
Warning labels: