Citrate buffer pH 6.0 (0.01 mol/l)
Citrate buffer pH 6.0 (0.01 mol/l) is used in medical diagnostics and histology to perform immunohistochemistry. In laboratories, it is used to control the acidic and basic reactions in solutions.
Chemically, the solution consists of aqua bidest, sodium hydroxide and citric acid. The citric acid and sodium hydroxide react to form sodium citrate and water, which contributes to the buffering effect. Sodium citrate can absorb and release protons, making it an effective buffer at pH 6.0.
Using the citrate buffer can lead to improved research results by creating a stable environment for chemical reactions and preventing unwanted pH fluctuations. As a result, molecules and structures of biological samples can be analyzed clearly and accurately.
The molarity of this solution is 0.01 mol/l, as indicated earlier. The osmolarity is about 0.02 osmol/l, based on the fact that sodium citrate dissociates completely in the solution. The buffer capacity depends on the concentration of the citrate ion and in this case is sufficient to maintain the pH around 6.0.
Chemically, the solution consists of aqua bidest, sodium hydroxide and citric acid. The citric acid and sodium hydroxide react to form sodium citrate and water, which contributes to the buffering effect. Sodium citrate can absorb and release protons, making it an effective buffer at pH 6.0.
Using the citrate buffer can lead to improved research results by creating a stable environment for chemical reactions and preventing unwanted pH fluctuations. As a result, molecules and structures of biological samples can be analyzed clearly and accurately.
The molarity of this solution is 0.01 mol/l, as indicated earlier. The osmolarity is about 0.02 osmol/l, based on the fact that sodium citrate dissociates completely in the solution. The buffer capacity depends on the concentration of the citrate ion and in this case is sufficient to maintain the pH around 6.0.
Article no.: 17885
Use as laboratory reagent
product information
Relevant Incredients:
• Sodium hydroxide
• Citric acid