Tri-sodium citrate buffer pH 6.0
Tri-sodium citrate buffer with a pH value of 6.0 is an important solution in various fields of application such as histology, biochemistry and molecular biology. It mainly consists of sodium citrate, a salt of citric acid, which can be found in various hydrate forms. By mixing sodium citrate and citric acid, the desired pH of 6.0 can be adjusted.
In histology, tri-sodium citrate buffer is often used as an antigen retrieval solution to improve epitope recognition during immunohistochemistry. In this method, the tissue is heated in the buffer, which partially breaks down the protein structures and makes the epitopes more accessible. This allows better binding of antibodies to their target structures and increases the sensitivity of the staining.
In biochemistry and molecular biology, tri-sodium citrate buffer is used as a buffer solution for enzyme reactions and in electrophoresis, among other applications. Its neutral to slightly acidic pH ensures stable conditions for many enzymes and enables the separation of proteins and nucleic acids in electrophoresis procedures.
Overall, Tri-sodium citrate buffer pH 6.0 is a versatile buffer solution used in various scientific disciplines to ensure optimal conditions for analyzing biological samples and performing biochemical experiments.
In histology, tri-sodium citrate buffer is often used as an antigen retrieval solution to improve epitope recognition during immunohistochemistry. In this method, the tissue is heated in the buffer, which partially breaks down the protein structures and makes the epitopes more accessible. This allows better binding of antibodies to their target structures and increases the sensitivity of the staining.
In biochemistry and molecular biology, tri-sodium citrate buffer is used as a buffer solution for enzyme reactions and in electrophoresis, among other applications. Its neutral to slightly acidic pH ensures stable conditions for many enzymes and enables the separation of proteins and nucleic acids in electrophoresis procedures.
Overall, Tri-sodium citrate buffer pH 6.0 is a versatile buffer solution used in various scientific disciplines to ensure optimal conditions for analyzing biological samples and performing biochemical experiments.
Article no.: 12442
Preparation of buffer solutions
product information
Relevant Incredients:
• tri-Sodium citrate dihydrate
• Hydrochloric Acid 37%