COOMASSIE solution 0.05% enables selective staining of proteins in polyacrylamide gels, making it indispensable for protein research. In COOMASSIE staining, the Coomassie Brilliant Blue R-250 dye binds to proteins and stains them intensely blue. The binding is based on an interaction between the dye and the amino acid side chains of the proteins.

The methanolic and acetic acid components of the solution facilitate the access of the dye to the proteins and assist in the removal of excess dye, resulting in increased contrast and enhanced visibility of the stained proteins.

Application of COOMASSIE solution enables quantitative analysis of protein amounts and discrimination of proteins based on their molecular size. In the context of COOMASSIE staining with GIEMSA, this solution is used to reveal protein distributions in biological samples.