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Biebrich’s solution

The Biebrich solution used in histology and scientific laboratories consists of Biebrich scarlet, acid fuchsin, Ponceau 2R, acetic acid and distilled aqua. / deionized water. It is an important component of staining kits, particularly trichrome for elastic fibers, and is used to distinguish collagen from muscle tissue in medical diagnostics.

Biebrich scarlet, a molecule discovered in 1878, is used to stain hydrophobic materials such as fat and oil. Acid fuchsin, a triphenylmethane dye, is required for Masson-Goldner trichrome staining and was used by Paul Ehrlich to stain acid-fast tuberculosis bacteria. Unfortunately, it has not been possible to find specific information on Ponceau 2R that is relevant in this context.

Using the Biebrich solution produces stains that show cell nuclei black, cytoplasm brown, muscle and myelinated fibers red, collagen blue, and erythrocytes scarlet.

Article no.: 18475

Staining of tissue samples

  •   Container size and order number Net price Amount
    Biebrich’s solution - 100 ml
    Order number: 18475.00100
    51,14 €
    Biebrich’s solution - 250 ml
    Order number: 18475.00250
    75,23 €
    Biebrich’s solution - 500 ml
    Order number: 18475.00500
    127,18 €
    Biebrich’s solution - 1.000 ml
    Order number: 18475.01000
    241,29 €

Important Information

UN-Nummer: 0000

Lagerung: 15 … 25 °C

Haltbarkeit: 6 Monate

safety data sheet


product information

Relevant Incredients:
• (C.I.: 26905)
• Acid Fuchsine (C.I.: 42685)
• Ponceau 2 R (C.I.: 16150)
• Acetic acid 99%

Technical informations

specifications

Hazard and safety instructions

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