Barbital-EDTA Buffer pH 8.6
Barbital EDTA Buffer pH 8.6 is used in medical and histological diagnostics for the preparation of buffer solutions. With sodium 5,5-diethylbarbiturate as the buffer component, it provides a stable environment for biological processes and interactions. In addition, EDTA, a strong chelating agent, enables the removal of metal ions from solutions, further increasing biochemical stability. Hydrochloric acid regulates pH to achieve optimal buffering capacity. The end result is a buffer solution that is ideal for a variety of histology and medical diagnostic applications.
Calculation of the molarity for sodium 5,5-diethylbarbiturate (M=236.27 g/mol) is approximately 0.0436 mol/l and for EDTA disodium salt dihydrate (M=372.24 g/mol) is approximately 0.00104 mol/l. The osmolarity is about 0.045 mol/l (assumption: no ion dissociation). The buffer capacity, defined by the ability of the buffer to keep the pH stable, is optimal in this case, since the pH is close to the pKs value of the barbital.
Calculation of the molarity for sodium 5,5-diethylbarbiturate (M=236.27 g/mol) is approximately 0.0436 mol/l and for EDTA disodium salt dihydrate (M=372.24 g/mol) is approximately 0.00104 mol/l. The osmolarity is about 0.045 mol/l (assumption: no ion dissociation). The buffer capacity, defined by the ability of the buffer to keep the pH stable, is optimal in this case, since the pH is close to the pKs value of the barbital.
Article no.: 16156
Preparation of buffer solutions
product information
Relevant Incredients:
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• EDTA
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