BRADFORD reagent (5x concentrate)
The solution consists of phosphoric acid (CAS no.: 7664-38-2), methanol (CAS no.: 67-56-1) and Coomassie Brilliant Blue G 250 (C.I.: 42655) (CAS No.: 6104-58-1).
The reaction that occurs when using the BRADFORD reagent is due to the interaction of the dye Coomassie Brilliant Blue G 250 with proteins. In the presence of proteins, the dye binds to them, shifting its absorption spectrum and developing a high absorption power in the region of 590-650 nm.
Chemical properties of the product result from the acidic solution provided by phosphoric acid and the solubility of the dye in methanol. During analysis, the amount of dye bound to proteins is determined by measuring the absorbance of the solution at a specific wavelength, which reveals the difference between bound and free dye.
Coomassie Brilliant Blue enters into a reaction with proteins, where it is indexed by the color change from brown to blue initiated by the proteins. Phosphoric acid provides the appropriate acidic pH in this process, while methanol acts as a solvent. The use of Coomassie Brilliant Blue benefits from its high affinity for proteins and its ability to form a strong Van der Waals bond.
The intensity of the color change can be used to draw conclusions about the concentration of proteins in the sample. This makes it possible to obtain an accurate overview of the protein content, allowing the reagent to be used, for example, in the diagnosis of diseases associated with altered protein levels.
Article no.: 18583
Protein staining
Important Information
Sales restriction: no private users!
UN-Nummer: 2924
Warning labels:
Lagerung: 15 … 25 °C
Haltbarkeit: 12 Monate
product information
Relevant Incredients:
• Phosphorsäure
• Methyl alcohol
• Coomassie brilliant blue G250 (C.I.: 42655)
Instructions / Protocol / Recommendations
Verwendung:
BRADFORD Reagent (5x Concentrate) is used for in vitro diagnostics of protein concentrations. Based on the Bradford method, a dye binding spectrophotometry, the dye Coomassie Brilliant Blue G 250 binds to proteins, resulting in a high absorbance in the range of 590-650 nm. The intensity of the color change allows conclusions to be drawn about the amount of protein in the sample. This is particularly useful in the diagnosis of diseases associated with altered protein levels. Phosphoric acid provides the acidic pH and methanol the solvent.
Prinzip:
The BRADFORD reagent is an in vitro diagnostic solution used to quantify protein concentrations in tissues or cells. The principle of operation is based on the binding of the dye Coomassie Brilliant Blue G-250 to proteins, resulting in a spectral shift in the absorbance of the dye. This shift is proportional to the amount of protein bound and can be analyzed photometrically. The actual amount of protein is determined using a suitable calibration curve. Phosphoric acid and methanol serve as solvents, while Aqua bidest (ultrapure water) dilutes the reagent and ensures the purity of the solution.
Verfahren:
The BRADFORD reagent is an in vitro diagnostic solution used to quantify protein concentrations in tissues or cells. The principle of operation is based on the binding of the dye Coomassie Brilliant Blue G-250 to proteins, resulting in a spectral shift in the absorbance of the dye. This shift is proportional to the amount of protein bound and can be analyzed photometrically. The actual amount of protein is determined using a suitable calibration curve. Phosphoric acid and methanol serve as solvents, while Aqua bidest (ultrapure water) dilutes the reagent and ensures the purity of the solution.
Hazard and safety instructions
| Signal Word 1: Gefahr |
| Signal Word 2: flamme, acid_red, exclam, silhouete |
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