Alcian Blue 8GS (CI no. 74240) – Powder
Alcian Blue 8GS is a cationic dye available in powder form. The dye is widely used in histological and microscopic applications to stain acidic polysaccharides, glycosaminoglycans and glycoproteins containing sulfate and/or carboxyl groups.
Alcian blue binds to these acidic structures, producing an intense blue hue. It is particularly used for staining mucins and acidic mucus in tissue sections. Alcian blue is also an important component of various multicolor staining protocols, such as Alcian blue/PAS staining for simultaneous visualization of neutral and acidic mucus.
This product is offered in a high-purity grade and conforms to CI No. 74240, and is suitable for a variety of research and diagnostic applications. The exact concentration and application should be determined according to specific experimental needs.
Alcian blue binds to these acidic structures, producing an intense blue hue. It is particularly used for staining mucins and acidic mucus in tissue sections. Alcian blue is also an important component of various multicolor staining protocols, such as Alcian blue/PAS staining for simultaneous visualization of neutral and acidic mucus.
This product is offered in a high-purity grade and conforms to CI No. 74240, and is suitable for a variety of research and diagnostic applications. The exact concentration and application should be determined according to specific experimental needs.
Article no.: 12529
Detection of mucopolysaccharides
product information
Relevant Incredients:
• Alcian blue 8GS (C.I.: 74240)
Instructions / Protocol / Recommendations
Verwendung:
Alcianblau wird in der Histologie verwendet, um sauro Mukosubstanzen (saure Mucine und sulfatierte Glykosaminoglykane wie Hyaluronsäure oder Chondroitinsulfat) bei niedrigem pH-Wert (1,0 bis 2,5) selektiv anzufärben. Je nach pH-Wert der Färbelösung können spezifische Mucine nachgewiesen werden. Eine weitere Methode der selektiven Darstellung, ist die Variation der Elektrolytkonzentration. Mit steigender Ionenstärke wird eine Färbung mit Alcianblau verhindert, wodurch eine Differenzierung unterschiedlich stark sulfatierter Mucopolysaccharide sowie Heparine, Heparinsulfate und Keratinsulfate möglich ist.