Alcian Blue 0.5 % (pH 2,6 with Acetic Acid)
Alcian Blue 0.5% (pH 2.6 with acetic acid) is primarily known for its use in histology to stain acidic mucins and sulfated glycosaminoglycans. The main component, alcian blue 8GS, binds to these structures to produce a distinct cyan stain.
Adjusting the pH with acetic acid to 2.6 is critical for specific binding of the dye to acidic mucins. These substances are polyanionic at pH 2.6 and interact strongly with the cationic Alcian blue. At this specific pH, the dye molecule is effectively converted to the cationic form, which promotes binding to acidic mucins.
The selective binding of the dye results in a high contrast between the stained structures and the surrounding tissue, allowing detailed examination of the morphology of these components. This precise staining pattern contributes to accurate histological analysis and diagnosis of various diseases associated with alterations in mucins and glycosaminoglycans.
Adjusting the pH with acetic acid to 2.6 is critical for specific binding of the dye to acidic mucins. These substances are polyanionic at pH 2.6 and interact strongly with the cationic Alcian blue. At this specific pH, the dye molecule is effectively converted to the cationic form, which promotes binding to acidic mucins.
The selective binding of the dye results in a high contrast between the stained structures and the surrounding tissue, allowing detailed examination of the morphology of these components. This precise staining pattern contributes to accurate histological analysis and diagnosis of various diseases associated with alterations in mucins and glycosaminoglycans.
Article no.: 10213
Detection of mucopolysaccharides
product information
Relevant Incredients:
• Acetic acid 99%
• Alcian blue 8GS (C.I.: 74240)
Instructions / Protocol / Recommendations
Verwendung:
Alcianblau wird in der Histologie verwendet, um sauro Mukosubstanzen (saure Mucine und sulfatierte Glykosaminoglykane wie Hyaluronsäure oder Chondroitinsulfat) bei niedrigem pH-Wert (1,0 bis 2,5) selektiv anzufärben. Je nach pH-Wert der Färbelösung können spezifische Mucine nachgewiesen werden. Eine weitere Methode der selektiven Darstellung, ist die Variation der Elektrolytkonzentration. Mit steigender Ionenstärke wird eine Färbung mit Alcianblau verhindert, wodurch eine Differenzierung unterschiedlich stark sulfatierter Mucopolysaccharide sowie Heparine, Heparinsulfate und Keratinsulfate möglich ist.