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Alcian Blue 0.2 %

Alcian Blue 0.2% (pH 2.5 with acetic acid) is a staining solution used in histology and cytology for staining acidic mucopolysaccharides and glycosaminoglycans. Alcian blue is a basic dye that selectively binds to acidic polysaccharides, highlighting them in a blue hue.

The solution is adjusted to a pH of 2.5 with acetic acid to optimize the selectivity of the stain. At this pH, Alcian blue binds primarily to acidic mucopolysaccharides, while other cell structures remain in the background. This allows for differential visualization and analysis of acidic mucopolysaccharides and glycosaminoglycans in cell and tissue samples.

Staining with Alcian Blue 0.2% (pH 2.5 with acetic acid) is used primarily in the study of connective tissue, cartilage, mucosa, and other tissues in which acidic mucopolysaccharides and glycosaminoglycans play an important role. The technique is particularly useful for examining the spatial distribution and quantity of these structures, which is relevant for assessing tissue function, structure, and possible disease.

Alcian blue can also be combined with other staining methods to provide multiple staining of different cellular structures and tissue components.

Article no.: 11836

Detection of mucopolysaccharides

  •   Container size and order number Net price Amount
    Alcian Blue 0.2 % - 100 ml
    Order number: 11836.00100
    24,52 €
    Alcian Blue 0.2 % - 250 ml
    Order number: 11836.00250
    27,98 €
    Alcian Blue 0.2 % - 500 ml
    Order number: 11836.00500
    37,81 €
    Alcian Blue 0.2 % - 1.000 ml
    Order number: 11836.01000
    68,87 €

Important Information

UN-Nummer: 0000

Lagerung: 15 … 25 °C

Haltbarkeit: 24 Monate

safety data sheet


product information

Relevant Incredients:
• Alcian blue 8GS (C.I.: 74240)
• Acetic acid 99%

Technical informations

specifications

Instructions / Protocol / Recommendations

Verwendung:

Alcianblau wird in der Histologie verwendet, um sauro Mukosubstanzen (saure Mucine und sulfatierte Glykosaminoglykane wie Hyaluronsäure oder Chondroitinsulfat) bei niedrigem pH-Wert (1,0 bis 2,5) selektiv anzufärben. Je nach pH-Wert der Färbelösung können spezifische Mucine nachgewiesen werden. Eine weitere Methode der selektiven Darstellung, ist die Variation der Elektrolytkonzentration. Mit steigender Ionenstärke wird eine Färbung mit Alcianblau verhindert, wodurch eine Differenzierung unterschiedlich stark sulfatierter Mucopolysaccharide sowie Heparine, Heparinsulfate und Keratinsulfate möglich ist.

Hazard and safety instructions

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