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Acetate Buffer 0.1 mol/l, pH 5.0

Acetate buffer pH 5.0 (0.1 mol/l) is used in biochemistry and molecular biology, especially in enzymatic reactions, DNA purification and immunoassays. It is suitable for keeping the pH value stable, an important property since enzyme activities are strongly influenced by pH fluctuations. When acetate buffer is used, hydrogen ions (H+) and acetate ions (CH3COO-) are formed. The ability of the buffer to absorb or release hydrogen ions without significantly changing the pH allows the specific pH environment to be maintained. This ensures consistent experimental conditions, which in turn allows reproducible results.

The buffer has a concentration of 0.1 mol/l. The osmolarity, derived from the concentration of dissolved particles, is 0.2 osmol/l, since both sodium acetate and acetic acid each contribute an osmotically active particle. The buffer capacity likewise at about 0.1 mol.

Article no.: 15656

Use as laboratory reagent

  •   Container size and order number Net price Amount
    Acetate Buffer 0.1 mol/l, pH 5.0 - 100 ml
    Order number: 15656.00100
    13,77 €
    Acetate Buffer 0.1 mol/l, pH 5.0 - 250 ml
    Order number: 15656.00250
    17,19 €
    Acetate Buffer 0.1 mol/l, pH 5.0 - 500 ml
    Order number: 15656.00500
    23,10 €
    Acetate Buffer 0.1 mol/l, pH 5.0 - 1.000 ml
    Order number: 15656.01000
    27,54 €
    Acetate Buffer 0.1 mol/l, pH 5.0 - 2.500 ml
    Order number: 15656.02500
    48,02 €

Important Information

UN-Nummer: 0000

Lagerung: 15 … 25 °C

Haltbarkeit: 12 Monate

safety data sheet

product information

Relevant Incredients:
• Sodium acetate tri-hydrate
• Acetic acid 99%

Technical informations

specifications

Hazard and safety instructions