Established Methods of Resin Histology

Technovit® 9100 – Histology and Immunohistochemistry

Technovit® 9100 is a plastic embedding system based on MMA (Methyl methacrylate). Technovit® 9100 is used in medicine, botany and zoology.

Technovit® 9100 was specifically developed for the embedding of mineralised tissues as well as soft tissue with an expanded study spectrum in light microscopy. The deplasticized sections are suitable for histological overview staining, enzyme chemistry and immunohistological studies, including in-situ hybridisation.

Thin sections for immunohistology can be stucked to glass object holders and deplasticized.

Technovit® 9100

Fields of application

  • Hard-cutting technique for making thin layers
    Examples: Iliac crest biopsies, smaller, spongy and compact bone tissue specimens
  • Division thin section technique (division procedure in point contact technology)
    Examples: Tooth/jaw areas with and without implants, non-cemented endoprostheses with shaft bones
  • Combined division-thin section technique and hard-cutting technique (target preparation)
    Examples: Boundary layer and environment assessment for metal implants and non-cemented endoprostheses

Tissues that cannot be cut are teeth-bearing jaw sections with fillings, crowns and bridges, thick corticalis, implant-bearing (metal or ceramic) jaw or long bones, or brittle, hypermineralised bones.

 

Material properties
Polymerisation of the hydrophobic Technovit® 9100 occurs by excluding oxygen using a catalyst system made of peroxide and amine. Additional components such as PMMA powder and regulator allow for a controlled polymerisation in the cold (in the range of -2 to -20 °C, depending on the volume) that guarantees complete dissipation of the polymerisation heat.

 

The benefits of the system at a glance

  • Polymerisation below freezing
  • Reproducibility of the embedding results and reliability due to constant, documented quality controls
  • Equal block hardening
  • The PMMA block remains transparent
  • Better results with regard to cutting and staining because Technovit® 9100 contains a hydrophilising agent
  • Can be used for thin section and the sawing and cutting techniques
  • Enzyme histology and immunohistology as well as in-situ hybridisation possible (sections)

Product data

Item No.DesignationQuantity
64715444 Technovit® 9100 Combipack1 x 1000 ml basic solution
1 x 120 g PMMA powder
8 x 1 g hardener 1
1 x 10 ml hardener 2
1 x 5 ml regulator 
66006735Technovit 9100 basic solution5000 ml
66010251 Technovit 9100 PMMA powder1000 g 
64709022Technovit 9100 hardener 1100 x 1 g
66039185 Technovit 9100 hardener 29 x 10 ml 
66039184Technovit 9100 regulator12 x 5 ml

 

The components

  • Technovit® 9100 basic fluid - component (1): The Technovit® 9100 basic solution is comprised of stablised methyl methacrylate. The hydrophily is improved through the addition of a suitable hydrophily agent. Technovit® 9100 basic solution can be used when stabilised and unstabilised.
  • Technovit® 9100 PMMA powder - component (2): The PMMA powder is used to guarantee a clear decrease in polymerisation shrinkage, a reduction in the polymerisation heat released and a better polymerisation process.
  • Technovit® 9100 hardener - component (3): Hardening powder 1 is a peroxide compound that starts polymerisation with hardener 2.
  • Technovit® 9100 hardener 2 - component (4): Hardening liquid 2 acts as a catalyst for hardener 1 to facilitate targeted polymerisation even at very low temperatures [< 0 °C].
  • Technovit® 9100 regulator - component (5): This is comprised of a reactive organic compound that facilitates a regulated polymerisation with controlled low temperature tops even for large quantities of polymerisation.

 

 

DesignationQuantityComponents
Technovit® 9100 basic solution stabilised 1 x 1000 ml
Technovit® 9100 PMMA powder120 g2
Technovit® 9100 hardener 1 8 bags, each 1 g
Technovit® 9100 hardener 210 ml4
Technovit® 9100 regulator 5 ml
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Fax 069 / 400 3019 64

Stains & Dyes
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Histology/Immunohistochemistry
Fon. 069 / 400 3019 - 65

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