Established Methods of Resin Histology

Technovit® 7100 – Sektions for Histology and Industrial Applications

Technovit® 7100 is a plastic embedding system based on HEMA* (2-hydroxyethyl methacrylate). The hydrophillic resin is used in medicine, botany, zoology and in the industry for embedding tissues for light microscope studies. The sections can be used for histological staining and enzyme detection.

Technovit® 7100 transparently polymerises. Uniform thin sections can be made out of the blocks with the rotation microtome. It is not necessary and also not possible to elute the plastic out of the block and the section.

Technovit® 7100

Material properties

The chemical polymerisation of Technovit® 7100 is initiated using a barbituric acid derivative in combination with chloride ions and benzoyl peroxide. The catalyst system does not have any aromatic amines compared to traditional systems.

 

Overview of the benefits

  • Uncomplicated handling
  • Reproducibility and reliability of the embedding due to the constant, documented quality controls of the individual components
  • Low polymerisation temperature due to Teflon embedding forms
  • Equal hardening of the block, thus uniform and thinnest possible sections
  • Low shrinkage artefacts, thus excellent tissue morphology
  • In addition to routine staining, enzyme detection is also possible
  • Less toxic due to catalyst made of barbituric acid
  • Polymerisation at room temperature (20 °C)
  • Airtight sealing not possible while hardening
  • Decalcifi cation not necessary for haematological illiac crest biopsies

 

Application

Prepare Technovit® 7100 in accordance with the step-by-step instructions. First place the fixated and dehydrated specimens in the pre-infiltration solution and then in the infiltration solution. Vacuum intervals and/or agitation during the individual steps accelerate the embedding process. Specimens for industrial application are placed directly in the infiltration solution or hardened without pre-treatment.

 

Polymerisation

Prepare the polymerisation mixture according to the instructions and then fill the embedding cavities with it. Immediately position the infiltrated specimens therein. Polymerisation occurs at room temperature.

 

Polymerisation temperature in:

  • Histoform S 32 °C – 38 °C
  • Histoform Q 40 °C – 45 °C

Block after hardening (approx. 1½ hrs.) with Histobloc® and Technovit® 3040.

 

Other fields of application

Technovit® 7100 is THE choice material for anything that pertains to making sections out of the embedded materials. The Technovit® 7100 - which was originally designed for histology - has proven itself in the industry for years due to its universal applicability. For example, the fi elds of application include embedding and sections made out of:

  • Plastics
  • Films
  • Paper
  • Textiles
  • Organic präparations
  • Fibres
  • Material properties

In cases where material specimens are cut and examined with a light microscope, embedding Technovit® 7100 facilitates sections in the U-area. The specimens can be economically cut in high quality with the Technovit Histoblade, a disposable blade coordinated with this system. 

These types of preparations are only slightly different from medicinal specimens. In many cases it is not necessary to place it in the preparation solution, as is the case with films and plastics.

 

Instructions for embedding non-medicinal tissue specimens

Infiltration in the preparation solution with vacuum is recommended for paper and textiles

Biological preparations are medically handled (fixed, dehydrated and subsequently infiltrated)

Materials that are less temperature-sensitive can also be embedded as large specimens (Histoform S and Q, or similar)

 

* HEMA is also known as GMA (Glycolmethacrylate)

© 2008 Morphisto GmbH | Home | Sitemap | Impressum | Print page
top

Contact

General Information
Fon. 069 / 400 3019 - 60
Fax 069 / 400 3019 64

Stains & Dyes
Fon. 069 / 400 3019 - 67

Histology/Immunohistochemistry
Fon. 069 / 400 3019 - 65

Contact form

Newsletter

News

no news in this list.